spider venom

The result of FCM showed the apoptosis rate of two kinds of cells changed after treatment with the spider venom ( 10,20,40 μ g/ mL) for 24 hours.

流式细胞仪检测发现经过雷氏大疣蛛蛛毒（10,20,40μg/mL）作用下24小时后两种细胞凋亡率增加。

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However, the spider toxin separated from the natural venom of spider venom is far from being able to meet the needs of the research and development. Acquisition of spider toxin has been a perennial restriction for spider toxin research.

然而从天然毒液中分离的蜘蛛毒素远远不能满足研究和开发的需要，蜘蛛毒素的获取一直是限制其研究的难题。

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So the researchers use spider venom protein to disrupt the channels and thus clarify exactly how the channels function.

因此研究人员用蜘蛛毒液干扰钠离子通道，因此恰好阐明了通道运作的原理。

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Spider venom protein messes with these sodium channels-that's why venom is dangerous.

研究发现蜘蛛毒液蛋白可以干扰这条彼此间的通道—这就说明毒液极具危险性的原因。

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RESULTS: The spider venom inhibited proliferation of BEL-7402 cells in time-dependent and concentration-dependent manners with 50% inhibitory concentration ( IC50) of 20?

结果雷氏大疣蛛蛛毒对BEL-7402细胞增殖有较强的抑制作用（P

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The result of this experiment showed that spider venom could inhibit the proliferation and DNA synthesis of BEL-7402.The possible mechanisms are to induce cell apoptosis and to down-regulated C-myc to change the cell cycle of BEL-7402 cells.

实验结果表明，敬钊缨毛蛛蛛毒可以抑制人肝癌细胞BEL7402的增殖和DNA的合成，其药理作用机理可能除了诱导凋亡外主要是使BEL7402细胞周期相关蛋白c Myc表达减弱，导致细胞周期的变化。

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In addition, 41 venomous proteins identified were of unknown function, and on average about half of high-quality MS/ MS spectra gave no signification search results, most likely due to the lack of a specific database for spider venom.

此外，在鉴定的毒素蛋白质中有41种功能未知。而且大约有一半以上的高质量的MS／MS数据得不到明显的鉴定结果，这很可能是没有特定的蜘蛛毒素蛋白质数据库的缘故。

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For those who study Biology, you may have brought the science behind my mom's cure: heat deactivates proteins and the spider's venom is simply a form of protein.

对于学生物学的人来说，你也许已经知道了我母亲这种治疗方式的科学依据：热量能够让蛋白质失去活性，而蜘蛛的毒液只是蛋白质组成的。

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Advance research and application of spider venom

蜘蛛毒素的研究概况及应用

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The technology of reverse phase chromatography is used in purification of spider venom mostly.

国内外在蜘蛛毒液的分离纯化上大多采用此种方法。

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The researchers recently asked for venom protein samples from Australian collaborators. And the Aussies accidentally included a spider venom protein that has no effect on humans or American cockroaches.

研究人员最近从澳大利亚合作研究人员那里找来毒液蛋白样品。然而他们意外发现这些样本中存在一个对人类和美国蟑螂没有影响的蜘蛛毒液蛋白样品。

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The spider venom also works to increase nitric oxide levels but through a different mechanism, Nunes said.

Nunes说，这种蜘蛛毒素也起增加一氧化氮水平的作用，但机理不同。

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